Klimša, V., Mašková, L., Kašpar, O., Ruphuy, G., & Štěpánek, F. (2023). Rapid screening of ternary amorphous formulations by a spray drying robot. International Journal of Pharmaceutics, International Journal of Pharmaceutics, Volume 651, 15 February 2024, 123739.

Klimša, V., Ruphuy, G., Jonáš, J., Mašková, L., Kašpar, O., Žvátora, P., & Štěpánek, F. (2023). Spray drying robot for high-throughput combinatorial fabrication of multicomponent solid dispersions. Powder Technology, 118872.

Tsagkaris, A. S., Louckova, A., Jaegerova, T., Tokarova, V., & Hajslova, J. (2022). The In Vitro Inhibitory Effect of Selected Asteraceae Plants on Pancreatic Lipase Followed by Phenolic Content Identification through Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS)International Journal of Molecular Sciences23(19), 11204.

Adrian, Edyta, et al. "Complexation of CXCL12, FGF-2 and VEGF with Heparin Modulates the Protein Release from Alginate Microbeads." International journal of molecular sciences 22.21 (2021): 11666.

Van Delft, F.C.M.J.M., Sudalaiyadum Perumal, A., Van Langen-Suurling, A., De Boer, C., Kašpar, O., Tokárová, V., Dirne, F.W.A., Nicolau, D.V., 2021. Design and fabrication of networks for bacterial computing. New Journal of Physics 23.

Mašková, L., Janská, P., Klimša, V., Knejzlík, Z., Tokárová, V., & Kašpar, O. (2021). Development of compartmentalized antibacterial systems based on encapsulated alliinase. Advanced Powder Technology.

Tokárová, V., Perumal, A. S., Nayak, M., Shum, H., Kašpar, O., Rajendran, K., ... & Nicolau, D. V. (2021). Patterns of bacterial motility in microfluidics-confining environments. Proceedings of the National Academy of Sciences118(17).

Crha, J., Basařová, P., Ruzicka, M. C., Kašpar, O., & Zednikova, M. (2021). Comparison of Two Solvers for Simulation of Single Bubble Rising Dynamics: COMSOL vs. Fluent. Minerals11(5), 452.

Janská, P., Knejzlík, Z., Perumal, A. S., Jurok, R., Tokárová, V., Nicolau, D. V., Štěpánek, F. & Kašpar, O. (2021). Effect of physicochemical parameters on the stability and activity of garlic alliinase and its use for in-situ allicin synthesis. Plos one16(3), e0248878.

2020 and older

Pittermannová A, Ruberová Z., Lizoňová D., Hubatová-Vacková A., Kašpar O., Zadražil A., Král V., Pechar M., Pola R., Bibette J., Bremond N., Štěpánek, F., Tokárová, V., Functionalized hydrogel microparticles prepared by microfluidics and their interaction with tumour marker carbonic anhydrase IX, Soft Matter (2020).

Kašpar, O., Koyuncu, A. H., Hubatová-Vacková, A., Balouch, M., & Tokárová, V. (2020). Influence of channel height on mixing efficiency and synthesis of iron oxide nanoparticles using droplet-based microfluidicsRSC Advances10(26), 15179-15189.

Held, M., Kaspar, O., Edwards, C., Nicolau, D.V., 2019. Intracellular mechanisms of fungal space searching in microenvironments. Proc Natl Acad Sci U S A 116, 13543-13552.

Kaspar, O., Koyuncu, A.H., Pittermannova, A., Ulbrich, P., Tokarova, V., 2019. Governing factors for preparation of silver nanoparticles using droplet-based microfluidic device. Biomed Microdevices 21, 88.

Perumal, A.S., Nayak, M., Tokárová, V., Kašpar, O., Nicolau, D.V., 2019. Space partitioning and maze solving by bacteria, Lecture Notes of the Institute for Computer Sciences, Social-Informatics and Telecommunications Engineering, LNICST, pp. 175-180.

Zuza, D., Soltys, M., Muzik, J., Lizonova, D., Lhotka, M., Ulbrich, P., Kaspar, O., Stepanek, F., 2019. Silica particles with three levels of porosity for efficient melt amorphisation of drugs. Microporous and Mesoporous Materials 274, 61-69.

Soltys, M., Balouch, M., Kaspar, O., Lhotka, M., Ulbrich, P., Zadrazil, A., Kovacik, P., Stepanek, F., 2018. Evaluation of scale-up strategies for the batch synthesis of dense and hollow mesoporous silica microspheres. Chemical Engineering Journal 334, 1135-1147.

van Delft, F., Ipolitti, G., Nicolau, D.V., Jr., Sudalaiyadum Perumal, A., Kaspar, O., Kheireddine, S., Wachsmann-Hogiu, S., Nicolau, D.V., 2018. Something has to give: scaling combinatorial computing by biological agents exploring physical networks encoding NP-complete problems. Interface Focus 8, 20180034.

Koyuncu, A.H., Kašpar, O., Ulbrich, P., Tokárová, V., 2019. Synthesis and characterization of nanoparticles using microfluidics, NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application, pp. 631-636.


Functional specimen


Czech Rise Up 2.0, projekt MPO 731745/2020 „Dezinfekční mikročástice s prodlouženým ochranným účinkem a metoda jeho vyhodnocení“
Replika prstu pro studium adheze antibakteriálních mikro částic na povrchu kůže - L. Večerková, J. Halberštát, K. Lukášová, L. Mašková, O. Kašpar, V. Tokárová


TAČR Zéta_TJ01000313-V1 „Vývoj biomimetických částic pro antibakteriální aplikace“
Model náplasti pro laboratorní testování antibakteriálních účinků - V. Tokárová, O. Kašpar, P. Jánská, M. Novák


Proven technology


Production and use of a polymer surface for non-enzymatic collection of adherent cells

ID RIV/60461373:22340/21:43923379

PrimeCell Advanced Therapy, a.s.; VŠCHT Praha



The subject of this proven technology is the technology of production and use of a polymer surface for enzymatic collection of adherent cells. The polymer surface was designed to allow cells to be cultured according to conventional culture procedures while providing users with the ability to separate cultured cells of adherent lines from the substrate by changing the temperature without the need for enzymes, dissociation buffers or mechanical harvesting methods. The final product of the proven technology is a carrier with a temperature-responsive substrate.




Device for continuous spray drying with variable inlet composition, method for continuous production of multi-component powders by spray drying and use thereof

European patent No. EP4039348

Klimsa, Vojtech; Ruphuy Chan, Gabriela; Stepanek, Frantisek; Kaspar, Ondrej



The present invention relates to a method for the fast and highly efficient production of multicomponent powders by spray drying. The method can be applied to already existing small-scale spray dryers available in the market and enables the production of multiple samples with variable composition in continuous mode. The present invention further relates to an automated device adaptable to small-scale spray drying equipment, consisting of an inlet pumping station 1, a cleaning mechanism 2 for the cyclone, a valve 3 adaptable to the cyclone, an autosampler 4, analyser 5, and an automatic control device 6 for the whole apparatus.